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Only lyophilised (powder) form should be stored frozen

Additional Wnt3a results in indefinite growth of mouse intestinal organoids devoid of a functionally intact Wnt-secreting niche, necessitating the addition of exogenous Wnt3a to the expansion medium and the exclusion of Wnt3a from the differentiation medium

Once reconstituted, the liquid solution must be refrigerated at 4C

Blood 107:508513 Reyland ME (2009) Protein kinase C isoforms: multi-functional regulators of cell life and death

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