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On the first day, all mice groups were fed a low-choline diet (choline bitartrate 0.08%), and fecal samples from each experimental group were collected and stored at 80 C for subsequent gut microbiota typing analysis

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recommended storage below -18C desiccated, with reconstituted solutions viable at 4C for 27 days

As such, the HGF binding properties of a selection of analogs with varying biological activity was assessed to determine if there was a relationship between inhibitory activity and affinity for HGF

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