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The dissociated single cells were seeded on AggreWell TM 800 plates (Stemcell Technologies, USA #34850) containing 300 microwells per well, pretreated with AggreWell rinsing solution (Stemcell Technologies, USA #07010), according to the users manual

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10.3389/fnins.2020.00564 100 JasiskaM.MiekJ.CymermanI

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The removal of superoxide radicals by SOD prevents the formation of the much more damaging peroxynitrite ONOO (O 2 + NO ONOO ) and maintains the physiologically relevant level of nitric oxide (NO ), an important molecule in neurotransmission, inflammation, and vasodilation