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GHK-Cu is administered as a daily subcutaneous injection with a 30-day cycle followed by a 30-day break

it is expressed at much lower levels in the cells in the colon than in the small intestine, where folate absorption primarily occurs 276

Fukui, S., Schwarcz, R., Rapoport, S

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Under conditions of compromised mitochondrial function, the PMF falls below the threshold required for ATP synthesis and the activity of ATP synthase can actually reverse such that it hydrolyzes ATP to pump protons back into the inner membrane space