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After staining, the cells were washed with PBS and subsequently imaged using fluorescence microscopy

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Patients may experience mild to moderate gastrointestinal symptoms, including nausea, reduced appetite, and occasional loose stools

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The samples were centrifuged at 12,000 rpm for 15 minutes

The outcome variable was total testosterone concentration, measured in ng/dL using isotope dilution liquid chromatographytandem mass spectrometry (ID-LCMS/MS) on serum specimens at the Division of Laboratory Sciences, National Center for Environmental Health (CDC method No