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Compared with the rats treated with APAP alone, rhein treated animals demonstrated significant reduction of the biochemical indicators of liver injury, including aspartate aminotransferase (AST), alanine aminotransferase (ALT), urea nitrogen (UREA), creatinine (CREA), nitric oxide (NO), and malondialdehyde (MDA)

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Then, 1% thiobarbituric acid was added to the clarified homogenate and incubated at 90 C for 1 h, followed by addition of 250 L of n-butanol

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