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To provide a more thorough theoretical foundation for treating disorders associated to TMAO, larger-scale clinical trials are needed to validate TMAOs potential as a biomarker for various diseases along the gut-organ axis

Serum triglyceride contents were determined using the Triglyceride Liquid Assay (Sentinel Diagnostics) following manufacturers instructions

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Antioxidative process involves the removal of excess ROS in the body in order to prevent oxidative stress from damaging cells and secondary apoptosis induced by oxidative stress (126, 127)