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She started the medication but: Wasn't sure if she was on the right dose Had no guidance on what to eat Wasn't losing weight like her neighbor on the same medication Had no way to ask questions when side effects occurred Felt completely lost and in the dark This isn't just frustratingit's potentially wasteful and ineffective

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Then, 5 l of Annexin VFITC and 5 l of propidium iodide (PI) were added, and the cells were gently vortexed