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In some particular embodiments of the present invention, the recrystallizing in the fragmental synthesis of the Lys 26 side chain in the preparation method is performed with one or a mixture of two or more of Et 2 O, CH 2 Cl 2 , CHCl 3 , MeOH, CH 3 CN, THF, EtOAc, EtOH, Toluene, Hexane, or Acetone, preferably a mixed solvent of MeOH and CH 2 Cl 2

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Figure 4, Panel B, presents a forest plot of HRs for CV mortality, illustrating a numerically lower point estimate for tirzepatide compared to the GLP-1RA class