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Due to both the high abundance of GSH and the millimolar K d value, we observed F 405 /F 488 changes only when RT reacted with GSH but not other thiols, reactive oxygen or nitrogen species (ROS/RNS) at their corresponding physiological concentrations (Fig

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10.3390/biology10111165 [DOI] [PMC free article] [PubMed] [Google Scholar] Tsuru-Aoyagi K., Potts M