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Nature 425 , 577584 (2003)

Liang D, Feng Y, Zandkarimi F, Wang H, Zhang Z, Kim J, et al

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[3] Cell Line:insulin + palmitic acid-induced insulin-resistant HepG2 cells, primary mouse hepatocytes, C2C12 mouse skeletal muscle myotubes Concentration:0, 20, 40, 80 M Incubation Time:24 h (following 24 h insulin + PA pre-treatment) Result:Significantly increased phosphorylation of p-IGF-1R (Tyr1135), p-IRS1 (Tyr612), p-PI3K, and p-Akt (Ser473) in a dose-dependent manner relative to insulin + PA-only treated cells across all three cell types, with significant increases observed at 20, 40, and 80 M

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