The residue was then homogenized in suspension with isolation buffer (10% w/v) using a hand-held glass homogenizer
Participants with normal hepatic function were eligible for inclusion if they were judged to be of general good health by the investigator, based on a medical examination (including physical examination, medical history, vital signs and ECG) and had liver enzyme levels within prespecified limits (alanine aminotransferase upper limit of normal [ULN] + 10%, aspartate aminotransferase ULN + 20% and total bilirubin ULN + 20%)
This method involves anchoring the C-terminal amino acid to a solid support and then sequentially adding the remaining amino acids
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While they will benefit your body, its important that you use them in conjunction with a healthy eating plan and consistent exercise routine