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Two independent experimental batches were used, and batch was included in all models as a fixed effect along with its interactions with treatment and age

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Our CLP model showed chronic Nrf2/HO-1/GPX4 suppression (day 7) linked to GSH depletion, ferroptosis, and irreversible antioxidant collapse (Xie et al., 2020), supporting Nrf2 activators (e.g., sulforaphane) or GSH precursors (e.g., NAC) as interventions (Yusri et al., 2025)

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