After three washes with TBST, membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (Jackson Immunoresearch, Pennsylvania, USA) for 1.5 h at room temperature
Borner T, Geisler CE, Fortin SM, Cosgrove R, Alsina-Fernandez J, Dogra M, Doebley S, Sanchez-Navarro MJ, Leon RM, Gaisinsky J, et al
Primary risks include additive cardiovascular effects (increased heart rate and blood pressure), intensified gastrointestinal symptoms (nausea, vomiting, diarrhea), and potential for serious adverse events including pancreatitis and acute kidney injury requiring close monitoring
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Insulin-like growth factor 1 (IGF-1) signaling in glucose metabolism in colorectal cancer
C.DukhandeV