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ALTERATIONS IN GLUTATHIONE PEROXIDASE AND SUPEROXIDE DISMUTASE ACTIVITIES IN PLASMA AND SALIVA IN RELATION TO DISEASE ACTIVITY IN PATIENTS WITH CROHN'S DISEASE INTRODUCTION Crohn's disease (CD) is a chronic inflammatory disorder of the entire gastrointestinal (GI) tract, which most commonly affects the terminal ileum and proximal colon (1-3)

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GSH-pull down assay 100 l of Glutathione Sepharose 4B beads (GE Health Life Science) were washed 3 times with 500 l of TNM buffer containing protease inhibitor cocktail tablets (Roche) before incubation with the protein samples

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They help stabilize cell membranes by interacting with lipid bilayers and minimizing lipid peroxidation