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As of June 2026, the FDA is in a public comment period on a proposal to formally exclude semaglutide, tirzepatide, and liraglutide from the 503B Bulk Drug Substances List

2.8 Elevated plus maze test The EPM test was implemented to investigate anxiety-like behavior in rodents, following the protocol delineated by Handley and Mithani (Guo et al., 2023)

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Given that isoelectric focusing necessitates the free migration of proteins based on their charge properties within an electric field, lower concentrations of polyacrylamide gels (e.g., 4%) are typically used to prevent molecular sieving