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The eluted sample was quickly neutralized by addition of 1/10 volume of 1 M Tris pH 8.0 and further polished on a size-exclusion chromatography column (Superdex 75, GE healthcare) pre-equilibrated with phosphate-buffered saline (PBS, pH 7.4)

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Understanding the frequency, management strategies, and warning signs of serious complications helps patients and healthcare professionals optimise treatment outcomes whilst minimising discomfort

The authors suggest that FFA3 might act to prevent hyperphagia-induced over-secretion of insulin that would cause beta cell exhaustion during dietary-related stress (Priyadarshini et al., 2020)

Some patients also benefit from creatine supplementation, which has an excellent safety profile and supports muscle maintenance

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