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Quenching of the fluorescence signal of the coumarin-labeled IR protein was analyzed with the equation: Allosteric binding of ITP and IR Competitive binding assay 57 Primary BMECs were pre-seeded in 12-well plates (2 10 5 per well) and cultured at 37 C for 24 h

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In addition, compared with wild-type mice, ethanol-fed LCN knockout mice had significantly reduced serum transferase, and significantly improved inflammation and steatosis (Moschen et al., 2017)

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