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3 Clinical implications and treatment perspectives Recent data support integrating routine metabolic screening, such as fasting blood glucose, fasting insulin, and homeostatic model assessment of IR (HOMA-IR), into standard BD care to identify high-risk individuals

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20 ng of cDNA was used per qPCR reaction using Powerup SYBR green master mix (Thermo Fisher Scientific) and gene specific primers according to manufacturers instructions on a Quantstudio 3 Real-Time PCR system (Thermo Fisher Scientific)

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Translation to human clinical application has been narrower than the breadth of the preclinical literature would suggest

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