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In humans, variations in IGF-1 levels and signaling have been linked to aging-related diseases

Interestingly, IGF-I is somehow related to all of them (Figure3)

Blots were blocked with phosphate-buffered saline with 0.1% Tween 20 and then bound with antibodies for Biotin, after which an enhanced chemiluminescence (ECL) reaction was performed for blot development

To compare the mean of the quantitative variables and the mean of their changes between the two groups at the beginning and conclusion of the research, an independent sample t -test was utilized

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