Pressure will be applied to the injection site after we remove the needle
Allylamine Allylamines, such as naftifine, butenafine, and terbinafine, inhibit a flavine adenine dinucleotide dependent enzyme, squalene epoxidase (EC 1.14.99.7) [171], responsible for converting squalene to squalene-2,3-epoxide, an intermediate in the biosynthesis of lanosterol [163, 172]
Njalsson R, Ristoff E, Carlsson K, Winkler A, Larsson A, Norgren S: Genotype, enzyme activity, glutathione level, and clinical phenotype in patients with glutathione synthetase deficiency
This internalization is a crucial step that then triggers the activation of the VEGFR2-Akt-eNOS signaling pathway
PMID: 10440166
We will also perform Bugbuster protein extraction followed by SDS-PAGE to assess the level of expression for the three glutathione enzymes