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Benfenati E, Gini G, Hoffmann S and Luttik R 2010 Comparing in vivo , in vitro and in silico methods and integrated strategies for chemical assessment: Problems and prospects

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The copper concentration used in experiments critically influences cellular responses: low levels (0.10.1.2mmol/l) induce physiological/adaptive transcriptional changes, while higher levels (0.4-0.4.6mmol/l) trigger toxic, stress-related responses [24]