The protein fractions were then loaded on the Step-affinity column equilibrated with binding buffer (with adjusted imidazole composition) and eluted after washing with 50 mM D-(+)-Biotin (Thermo Scientific) after 1 h incubation on the bead
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9 Moreover, all athletes who underwent PRP injection managed to return to training or daily sports activity
DOI: 10.1002/jnr.490160211 Rizzoli SO, Betz WJ (2004): The structural organization of the readily releasable pool of synaptic vesicles
