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Utilizing physiologically relevant O 2 -, this approach mimics oxidative stress conditions with high sensitivity (Chiefa et al., 2024)

By administering the ferroptosis inducers RSL3 and IKE and knocking out the GPX4 gene in immortalized mouse fibroblasts, respectively, Kraft identified a common gene, GCH1, in the surviving cells by genetic sequencing [65]

Thus, in the well-fed state, insulin enhances the carbon flux through the pentose phosphate pathway, increasing NADPH production Note: insulin also promotes fatty acid synthesis, which depends on a continuous supply of NADPH

Glutathione is a small protein molecule that gets digested and lost in the digestive tract, making it ineffective at raising intracellular glutathione levels in the most important detoxification organs, such as liver, kidneys, and lungs