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After freeze-thaw processing, supernatants were collected and stored at -80C until required ( 2.2 Cell viability assay Cell Counting Kit-8 (CCK-8) (Beyotime Biotech, China) was used to measure the corresponding optical density ratio marker cell viability levels in 96-well plates according to the manufacturers instructions

They are vital in maintaining systemic iron equilibrium (Rochette et al., 2022)

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It involves direct engagement with industry participants to ensure accuracy and depth in analysis

Dosing: 150-500 mcg (0.15-0.5 mg) per day, administered intranasally or subcutaneously