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For heat-mediated antigen retrieval, tris-EDTA (pH 9.0) treatment was applied for 30 min, followed by a 5-min incubation with immunoblock (Epredia, TL-125-QHD)

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Alnylams patents on these specific lipid structures, combined with the specific lipid ratios and particle size specifications, constitute a delivery IP moat that is technically meaningful and legally defensible exactly the category that survives the post- Teva v

The review suggests their effects may be comparable, and because they use different mechanisms, combining them could provide additive benefits