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Proteins (1030 g/well, equal amounts within group but different amounts among groups for better comparison) were separated on 10% or 12% SDS-PAGE gel and transferred on an equilibrated polyvinylidene difluoride membrane (PVDF, Amersham Biosciences, Buckinghamshire, UK)

Mild headaches or discomfort can occur rarely, but it's generally well-tolerated

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Smith, S., Giriat, I., Schmitt, A., de Lange, T

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