The primers were annealed for 10 min at 25 C, reverse transcription was performed for 15 min at 42 C, and inactivation was performed for 5 min at 85 C
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Thus, in oocytes, maintenance of proper lipid oxidation with/without a minimum production of free radicals is crucial to preserve its quality [30]
Above all, a connection between B12 and MS is suspected, but so far insufficiently researched (2, 3)
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