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If your Zomacton vials are 5 mg, they can be mixed with 1 to 5 ml of bacteriostatic water

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Samples were washed and subsequently incubated with secondary antibody for 45 min at RT (Table 3) Sections were counterstained either with the diaminobenzidine substrate kit (Nacalai, USA) followed by hematoxylin, or sections were stained with 6-diamidino-2-phenylindole (DAPI, Thermo Fisher, USA) for visualization of cell nuclei as previously described

Specifically, the occurrence of peptide and protein aggregation in biological systems can cause diseases and in pharmaceutical drug products induce adverse immunological responses 10,11,12

Eppendorf cups were flushed additionally with 200 L to transfer remaining cells

PMID 17132851