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For glutamine treatment, FBS-supplemented culture media was removed and replaced with glutamine-pyruvate and sodium-pyruvate depleted XF DMEM media pH 7.4 (Agilent)

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You can rotate in any order you want: thigh to thigh, upper arm to stomach, stomach to thigh, and so on

Yet, molecular mechanisms underlining GLP-1 mediated stimulation of insulin secretion are still not fully characterized

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