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ABHD11-Flag protein was then eluted in 600 l 100 g ml 1 3X Flag peptide and rotated at 4 C for 1 h

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Peripheral blood mononuclear cells (190), thyroid lymphocytes (191), and lymph node lymphocytes demonstrated cell-mediated immunity to TPO, Tg and TSH-R (192, 193), and to specific epitopes of these molecules (194-199)