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Monitor pain using a 0-10 scale during and after activity Activity should not increase pain beyond baseline Post-activity soreness should resolve within 24 hours Watch for swelling or warmth returning to the injection site Pain escalating to 3-7/10 levels signals overload How Do You Increase Load Without Triggering A Flare-Up

Acknowledgements The research was conducted in the APC Microbiome Ireland which is funded by Science Foundation Ireland (SFI/12/RC/2273_P2)

Consequently, as also indicated in our other liver studies (Ilic et al., 2009

Practical Implication: This research validates that topical GHK-Cu formulations achieve meaningful tissue concentrations, supporting the rationale for both cosmetic and research applications

MMP, matrix metalloproteinases

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