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Repeated activation of the receptors can lead to upregulation and sensitization, causing hyperexcitability of the receptor even at low levels
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In situ hybridization was performed on the human liver biopsies on a Ventana Discovery Ultra automation system (Ventana Medical Systems) using an RNAscope VS Universal HRP/AP Kit (Advanced Cell Diagnostics) and the specific GLP1R probe, RNAscope 2.5 VS Probe-Hs-GLP1R (Advanced Cell Diagnostics, cat
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