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when the research objectives allow it, used non-lethal sampling techniques instead of lethal collecting, and used preferential collection of post reproductive individuals (or the life stage with the least reproductive value) when lethal collection is necessary to improve the chances of the species survival

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First, melatonin appears most useful where oxidative load is high (e.g., PCOS, diminished ovarian reserve, repeat-poor response), aiming to improve oocyte/embryo competence rather than to replace first-line ovulation-induction strategies