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Combining a powerful GLP-1 medication with a very-low-calorie diet (VLCD) can increase the risk of severe side effects like malnutrition, gallstones, and muscle loss

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Protein digestion by trypsin was performed according to the filter-aided sample preparation (FASP) procedure described by Matthias Mann ( Figure 1 2.6 LC-MS/MS analysis Labeled peptides were fractionated by High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific)

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