First, the mechanical agitation subjects peptide chains to shear forces at the liquid-air interface
Caki-1 cells were cultured in McCoys 5A +FBS [pyruvate-free McCoys 5A containing 16 mM glucose (ThermoFisher, 16600082) supplemented with 10% FBS and 1% penicillin/streptomycin] at 37C in 5% CO 2
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In addition to caffeine, coffee contains several phytochemicals that can influence health