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Cell lines and culture conditions HepG2 cells, a human hepatoma cell line, were obtained from the American Type Culture Collection (Manassas, VA) and were cultured in Dulbeccos modified Eagles medium (DMEM) containing 10% (v/v) fetal bovine serum, 2 mM glutamine, 5 U/mL penicillin, and 50 g/mL streptomycin at 37 C in a humidified O 2 /CO 2 (19:1) atmosphere

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Understanding the influence of dietary habits on autoimmune disorders has become increasingly critical, as the global prevalence of these conditions continues to rise and curative treatments remain limited

Due to a lack of scientific research, very little is known about the side effects of using glutathione supplementation

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