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The critical role of heavy metals in causing oxidative damage does suggest that this is a significant mechanism that is also involved in metal toxicity, and thus, this has prompted scientists to investigate the ability of various natural or synthetic antioxidants in preventing and attenuating metal-induced biochemical alterations

In the negative control, normal rabbit IgG (ab172730, Abcam, Cambridge, UK) diluted to 4 g/mL in 1% rabbit serum in PBS was used instead of the primary antibodies

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Sampling Intervals: 2hr,4hr,6hr,8hr,l0hr,l2hr and 24 hr

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