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The mRNA level of PFK , a key glycolysis-related gene, was significantly decreased (fasting state) and increased (feeding state) in the muscle, but was comparable in the liver (Fig

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The pipette (68 M) was pulled by a micropipette puller (P-1000, Sutter instrument) and filled with the internal solution that contained (in mM): 130 K-gluconate, 5 KCl, 4 Na2ATP, 0.5 NaGTP, 20 HEPES, 0.5 EGTA (PH 7.28, 290300 mOsm)

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