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X-tremeGENE HP DNA Transfection Reagent (Roche) was used for transient transfection, and the total amount of transfected DNA was normalized by addition of empty control plasmids

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A clinical review of recent findings in the epidemiology of inflammatory bowel disease

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Clinical research in athletic populations found that doses up to 6.4g daily were well-tolerated, with mild tingling sensations being the only reported side effect from beta-alanine precursor loading (Trexler et al., 2015)