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The free Ca 2+ concentrations of the lysis and stimulation buffer were estimated at 121 nmol/l (for 1.65 mmol/l CaCl 2 ) and 1.2 to 1.4 mol/l (for 6.65 mmol/l CaCl 2 ), respectively, using BAD4-software [25]
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Reconstitution simply means adding a sterile liquid to a lyophilized peptide powder so it can dissolve into a usable research solution
Bennett et al., 2013)
DOI: 10.1021/jm960840h Melanocortin ligands: 30 years of structure-activity relationship studies Medicinal Research Reviews, 2004
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