Actin was used as an internal reference gene
Acknowledgements The research conducted in this study received support from the collaborative project between National Tsing Hua University and Kaohsiung Medical University to Dr

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It doesn't happen instantaneously at a specific date it's a gradual degradation that accumulates over time with exposure to heat, moisture, light, and freeze-thaw cycles
doi: 10.1016/j.foodchem.2021.130670 57 SadasivamS.ManickamA
October 2023: Researchers conducted a large observational study of about 5,400 patients who took semaglutide, liraglutide, or bupropion-naltrexone for weight loss