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To evoke neuronal activation within the medial prefrontal cortex, a focal application of 1 mM NMDA was delivered to the cingulate cortex (AP, +2.04 mm, ML, +0.36 mm, and DV, 1.2 mm from the bregma) for 50 ms at 8 psi via a pressure-ejection system (Picospritzer II, Parker Hannifin, USA)

Instead, gently roll the vial between your fingers or swirl it slowly until the solution is completely clear

J Neurosci 28:50075017

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In another group of mice (ten mice/group), the survival time of each mouse was measured from the time point when intracranial injection of tumor cells was performed until the clear signs of morbidity appeared