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Twice-daily dosing is used in some early-phase research protocols to maintain more consistent receptor activation during the initial neuroplasticity induction period, but this approach increases total daily exposure and may elevate the risk of off-target effects

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Engel, L

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The OPLS-DA score plot (Fig

Metabolites extraction The chemicals used for LC-MS, including methanol, acetonitrile, deionized water (dH 2 O), and formic acid, were sourced from Fisher Scientific Company (Ottawa, ON, Canada)