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Samples were reduced with dithiothreitol at 10 mM for 30 minutes at 56C and alkylated with iodoacetamide at 30 mM for 45 minutes at room temperature in the dark

The effect of glutathione IV therapy is gradual

doi: 10.1038/nm.3361 96 PerkinsMNRothwellNJStockMJStoneTW