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After 48 h incubation of confluent monolayers with cARLA, the culture medium was removed and hECs were fixed with a 1:1 mixture of ice cold methanol-acetone solution for 2 min

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To understand the effect of host cell glutathione on existing infections, additional experiments were conducted in which A6 cells were supplemented with 2.5 mM cysteine at 24 hr post infection

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Loren Pickart and colleagues established GHK-Cu's role in wound healing and gene expression

However, the role of YAP/TAZ and Wnt signaling in intestinal self-renewal still needs further study