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"I was starting to feel like I was just this negative burden for everybody," she says

In some cases, providers may request additional information or decline treatment based on risk factors

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The assay was performed by heating samples in a Mx3005p qPCR machine (Agilent Technologies, USA) from 25 to 97 C in 1 C min 1 increments for 30 s in an expanding saw-tooth profile, a refinement of a previously described protocol 24