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High-protein, fiber-rich foods are recommended to support weight loss and reduce GI side effects

These can increase the risk for unpleasant complications and may also make weight loss harder

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To generate an amplicon library, 20 ng of each genomic DNA sample, 1.25 U of Taq DNA polymerase, 5 l of 10 Ex Taq buffer, 10 mM dNTPs (all reagents from TaKaRa Biotechnology Co., Ltd, Dalian, China), and 40 pmol of primer mix were added to a 50 l reaction mixture

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Notably, combination with anti-PD-1 checkpoint blockade yields synergy, significantly enhancing the ICB responsiveness of TNBC and producing improved therapeutic efficacy