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Results were normalized by total DNA or number of islets

10.3390/ijms21031047 41

The response begins within 10 min but is generally slower than cephalic phase events and peaks after an hour

First, the experimental design did not include a remogliflozin-only group, which limited our ability to distinguish the direct effects of remogliflozin from its interaction with TAA-induced pathology or potential vehicle effects

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The cells were rinsed with PBS three times, and images were captured using the EVOS FL auto imaging system (Thermo Fisher Scientific)