Bilateral NTS injection of TVA + RabiesG and counterbalanced unilateral injection of RABV (4 mice / side) resulted in 40.5% (5.5) of all PPG NTS neurons being successfully transduced starter neurons, identified by colocalisation of mCherry (and/or tdRFP) and GFP (photomicrographs in a-h representative of independent experiments from 8 animals)
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Real-time PCR was conducted for amplification with specific primers for the chondrogenic markers COL2A1 (Forward: 5-TTCAGCTATGGAGATGACAATC-3 and Reverse: 5-AGAGTCCTAGAGTGACTGAG-3), ACAN (Forward: 5-AGCCTGCGCTCCAATGACT-3 and Reverse: 5-TAATGGAACACGATGCCTTTCA-3), and SOX9 (Forward: 5-TTCCGCGACGTGGACAT-3 and Reverse: 5-TCAAACTCGTTGACATCGAAGGT-3)
O.BaeS.HongJ.et al (2022)
doi:10.1111/j.1460-9568.2004.03650.x Li Y, Maher P, Schubert D (1997) A role for 12-lipoxygenase in nerve cell death caused by glutathione depletion
cardiovascular and fibrosis endpoint studies favour semaglutide